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Enzymes for Research, Diagnostic and Industrial Use

α-L-Arabinofuranosidase B25 from Bacteroides ovatus, Recombinant

Cat No.
NATE-1026
Description
Alpha-N-arabinofuranosidase is an enzyme with system name alpha-L-arabinofuranoside arabinofuranohydrolase. This enzyme catalyses the following chemical reaction: Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides. The enzyme acts on alpha-L-arabinofuranosides, alpha-L-arabinans containing (1,3)- and/or (1,5)-linkages, arabinoxylans and arabinogalactans.
Abbr
α-L-Arabinofuranosidase B25, Recombinant (Bacteroides ovatus)
Source
Bacteroides ovatus
Applications
For use in plant cell wall carbohydrate and biofuels research.
Product Overview
High purity α-L-arabinofuranosidase (Bacteroides ovatus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
Solution containing 50% glycerol and 0.02% (w/v) sodium azide
Enzyme Commission Number
EC 3.2.1.55
Activity
25 U/mg protein (on wheat arabinoxylan) at pH 6.5 and 40°C
CAS No.
9067-74-7
Molecular Weight
~ 69,000
Isoelectric point
~ 6.0
pH Stability
5.0-9.0
Specificity
Hydrolysis of terminal, non-reducing a-L-arabinofuranose from singly substituted xylose residues in arabinoxylan (a-1,2 and a-1,3). Does not hydrolyse a-L-arabinofuranose from doubly substituted xylose residues in arabinoxylan.
Unit Definition
One Unit of α-L-arabinfuranosidase activity is defined as the amount of enzyme required to release one μmole of arabinose per minute from wheat arabinoxylan (10 mg/mL) in sodium phosphate buffer (100 mM), pH 6.5 at 40°C.
Optimum pH
6.5
Optimum temperature
40°C
Thermal stability
up to 50°C
Stability
> 4 years at -20°C.
Storage
Stored at -20°C
Preparation Instructions
For assay, this enzyme should be diluted in sodium phosphate buffer (100 mM), pH 6.5 containing 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Synonyms
EC 3.2.1.55; non-reducing end alpha-L-arabinofuranosidase; alpha-L-arabinofuranoside non-reducing end alpha-L-arabinofuranosidase; Arabinoxylan arabinofuranohydrolase axhA-2
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