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Enzymes for Research, Diagnostic and Industrial Use

α-L-Arabinofuranosidase from Ustilago maydis, Recombinant

Cat No.
NATE-1046
Description
Alpha-N-arabinofuranosidase is an enzyme with system name alpha-L-arabinofuranoside arabinofuranohydrolase. This enzyme catalyses the following chemical reaction: Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides. The enzyme acts on alpha-L-arabinofuranosides, alpha-L-arabinans containing (1,3)- and/or (1,5)-linkages, arabinoxylans and arabinogalactans.
Abbr
α-L-Arabinofuranosidase, Recombinant (Ustilago maydis)
Source
Ustilago maydis
Applications
For use in plant cell wall carbohydrate and biofuels research.
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.55
Activity
25 U/mg protein (on wheat arabinoxylan) at pH 5.0 and 40°C.
CAS No.
9067-74-7
Molecular Weight
~ 35,000
Isoelectric point
~ 8.4
pH Stability
3.0 - 9.0
Specificity
Hydrolysis of terminal, non-reducing a-L-arabinofuranose from singly substituted xylose residues in arabinoxylan (a-1,2 > a-1,3). Does not hydrolyse a-L-arabinofuranose from doubly substituted xylose residues in arabinoxylan.
Unit Definition
One Unit of α-L-arabinfuranosidase activity is defined as the amount of enzyme required to release one μmole of arabinose per minute from wheat arabinoxylan (10 mg/mL) in sodium acetate buffer (100 mM), pH 5.0 at 40°C.
Optimum pH
5
Optimum temperature
40°C
Thermal stability
up to 50°C
Stability
> 2 years at 4°C.
Storage
4°C
Preparation Instructions
For assay, this enzyme should be diluted in sodium acetate buffer (100 mM), pH 5.0 containing 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Synonyms
non-reducing end alpha-L-arabinofuranosidase; alpha-L-arabinofuranoside non-reducing end alpha-L-arabinofuranosidase; EC 3.2.1.55; arabinosidase; alpha-arabinosidase; alpha-L-arabinosidase; alpha-arabinofuranosidase; polysaccharide alpha-L-arabinofuranosidase; alpha-L-arabinofuranoside hydrolase; L-arabinosidase; alpha-L-arabinanase; Alpha-N-arabinofuranosidase; α-L-Arabinofuranosidase
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