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β-Mannosidase from Cellulomonas fimi, Recombinant

Cat No.
NATE-1115
Description
Beta-mannosidase is an enzyme with system name beta-D-mannoside mannohydrolase. This enzyme catalyses the following chemical reaction:Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides. This gene encodes a member of the glycosyl hydrolase 2 family. The encoded protein localizes to the lysosome where it is the final exoglycosidase in the pathway for N-linked glycoprotein oligosaccharide catabolism. Mutations in this gene are associated with beta-mannosidosis, a lysosomal storage disease that has a wide spectrum of neurological involvement.
Abbr
β-Mannosidase, Recombinant (C. fimi)
Source
Cellulomonas fimi
Applications
High purity recombinant beta-Mannosidase (C. fimi) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.25
Activity
13.2 U/mg protein (on pNP-β-D-mannopyranoside) at pH 6.5 and 35°C.
CAS No.
9025-43-8
Molecular Weight
~ 103,000
Isoelectric point
~ 4.8
pH Stability
4.0 - 9.0
Unit Definition
One Unit of β-D-mannosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from pNP-β-D-mannopyranoside (0.8 mM) in sodium maleate buffer (100 mM) pH 6.5 and 35°C, monitored at 400 nm.
Optimum pH
6.5
Optimum temperature
35°C
Thermal stability
up to 35°C
Stability
> 2 years at 4°C
Storage
4°C
Preparation Instructions
For assay, this enzyme should be diluted in sodium maleate buffer (100 mM), pH 6.5 containing 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Synonyms
β-mannosidase; mannanase; mannase; β-D-mannosidase; β-mannoside mannohydrolase; exo-β-D-mannanase; EC 3.2.1.25; 9025-43-8
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Realted Protocols
b-mannosidase -Enzymatic Assay Protocol
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