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β-N-Acetylhexosaminidase from Prokaryote, Recombinant

Cat No.
NATE-1043
Description
This enzyme releases non-reducing terminal β1-2, β1-3, β1-4 and β1-6 linked N-acetylglucosamine from complex carbohydrates. When incubated with oligosaccharides at low concentrations (<50 mU/ml) the enzyme can differentiate between GlcNAcβ1-2Man, GlcNAcβ1-4Man and GlcNAcβ1-6Man linkages. Under such conditions, the enzyme cleaves essentially only β1-2 linked GlcNAc, with two provisos. Firstly, β1-2 GlcNAc is not hydrolyzed if the mannose to which it is substituted has a substitution at C-6. Thus, the enzyme is useful for the analysis of tri-antennary oligosaccharides. Secondly, if the β-linked mannose of the conserved pentasaccharide core is substituted with a "bisecting" GlcNAc then only the β1-2 linked GlcNAc linked to mannose on the α1-3 arm is cleaved. At higher concentrations of the enzyme, β1-4 and β1-6 linked GlcNAc may also be hydrolyzed.
Abbr
β-N-Acetylhexosaminidase, Recombinant (Prokaryote)
Source
Prokaryote
Applications
For use in glycobiology research.
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.52
Activity
~ 260 U/mg protein (on pNP-β-D-N-acetylglucosamine) at pH 4.0 and 40°C; ~ 723 U/mg protein (on pNP-β-D-N-acetylglucosamine) at pH 4.0 and 60°C.
CAS No.
9012-33-3
Molecular Weight
~ 52,000
Isoelectric point
~ 6.3
pH Stability
3.0-9.0
Specificity
Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-β-Dhexosaminides from glycoproteins and oligosaccharides.
Unit Definition
One Unit of β-N-acetyl-D-hexosaminidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from pNP-β-D-N-acetylglucosamine (1 mM) in Citrate-phosphate buffer (100 mM) pH 4.0 at the temperatures indicated, monitored at 400 nm.
Optimum pH
4
Optimum temperature
60°C
Thermal stability
up to 50°C
Stability
> 2 years at 4°C.
Storage
4°C
Preparation Instructions
For assay, this enzyme should be diluted in Citratephosphate buffer (100 mM), pH 4.0 containing 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Synonyms
beta-N-acetyl-D-hexosaminide; N-acetylhexosaminohydrolase; β-N-Acetylhexosaminidase; N-Acetyl-β-D-glucosaminidase, β-N-Acetylglucosaminidase
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0779
EC No.EC 3.2.1.52
CAS No.9012-33-3
SourceBovine kidney
CatalogNATE-0780
EC No.EC 3.2.1.52
CAS No.9012-33-3
SourceCanavalia ensiformis
CatalogNATE-0781
EC No.EC 3.2.1.52
CAS No.9012-33-3
SourceHuman placenta
CatalogNATE-0782
EC No.3.2.1.52
CAS No.9012-33-3
SourceE. coli
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b-n-acetylglucosaminidase -Enzymatic Assay Protocol
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