Description
A thermostable Alpha-L-Rhamnosidase (Naringinase, RhamA) that catalyzes the cleavage of the bond between terminal L (+)-rhamnose and the aglycone of rhamnose-containing glycosides. The enzyme is very active on naringin but has also substantial activity with hesperidin as substrate.
Abbr
RhamA, Recombinant (Prokaryote)
Applications
High purity recombinant alpha-Rhamnosidase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.40
Activity
190 U/mg protein at pH 6.5 and 50°C; 135 U/mg protein at pH 6.5 and 40°C.
Molecular Weight
~ 75,400
Unit Definition
One Unit of α-L-rhamnosidase activity is defined as the amount of enzyme required to release one µmole of of p-nitrophenol (p-NP) per minute from p-nitrophenyl-α-Lrhamnoside (5 mM) in sodium phosphate buffer (100 mM), pH 6.5 at 50°C.
Thermal stability
up to 50°C
Stability
> 2 years at 4°C
Preparation Instructions
For assay, this enzyme should be diluted in sodium phosphate buffer (20 mM), pH 6.5 containing 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Synonyms
glycoside hydrolase; RhamA; naringinase; hesperidinase; α-L-rhamnosidase A; α-L-rhamnosidase N; α-L-rhamnoside rhamnohydrolase; EC 3.2.1.40
