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α-Rhamnosidase from Prokaryote, Recombinant

Cat No.
NATE-1124
Description
A thermostable Alpha-L-Rhamnosidase (Naringinase, RhamA) that catalyzes the cleavage of the bond between terminal L (+)-rhamnose and the aglycone of rhamnose-containing glycosides. The enzyme is very active on naringin but has also substantial activity with hesperidin as substrate.
Abbr
RhamA, Recombinant (Prokaryote)
Alias
RhamA
Source
Prokaryote
Applications
High purity recombinant alpha-Rhamnosidase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.40
Activity
190 U/mg protein at pH 6.5 and 50°C; 135 U/mg protein at pH 6.5 and 40°C.
CAS No.
37288-35-0
Molecular Weight
~ 75,400
Isoelectric point
~ 5.9
pH Stability
4.0 - 9.0
Unit Definition
One Unit of α-L-rhamnosidase activity is defined as the amount of enzyme required to release one µmole of of p-nitrophenol (p-NP) per minute from p-nitrophenyl-α-Lrhamnoside (5 mM) in sodium phosphate buffer (100 mM), pH 6.5 at 50°C.
Optimum pH
6.0 - 6.5
Optimum temperature
50°C
Thermal stability
up to 50°C
Stability
> 2 years at 4°C
Storage
4°C
Preparation Instructions
For assay, this enzyme should be diluted in sodium phosphate buffer (20 mM), pH 6.5 containing 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Synonyms
glycoside hydrolase; RhamA; naringinase; hesperidinase; α-L-rhamnosidase A; α-L-rhamnosidase N; α-L-rhamnoside rhamnohydrolase; EC 3.2.1.40
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0653
EC No.EC 3.2.1.40
CAS No.37288-35-0
SourceThermomicrobia strain PRI-1686
CatalogNATE-1164
EC No.EC 3.2.1.40
CAS No.37288-35-0
SourcePenicillium sp.
CatalogNATE-1510
EC No.EC 3.2.1.40
CAS No.37288-35-0
SourceE. coli
CatalogEXWM-3903
EC No.EC 3.2.1.40
CAS No.37288-35-0
Source
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Realted Protocols
HESPERIDINASE -Enzymatic Assay Protocol
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