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β-Xylosidase, thermostable, Recombinant

Cat No.
NATE-0789
Description
Releases reducing sugars from birchwood xylan (X0502), also catalyzes the hydrolysis of 4-methylumbelliferyl-β-D-cellobioside and 4-methylumbelliferyl-β-D-glucopyranoside. This enzyme does not possess endo-xylanase, arabinoxylanase or β-glucanase activities. β-Xylosidase undergoes post-translation glycosylation which has been shown to be critical for its proper activity and stability. Deglycosylation altered the the optimum temperature and pH for activity and decreased its thermostability.
Abbr
β-Xylosidase, Recombinant
Alias
β-Xylosidase
Source
E. coli
Form
liquid, Supplied as a solution in 50 mM Tris-HCl, pH 8.0, 100 mM NaCl, and 25% glycerol.
Activity
> 35 units/mg protein
CAS No.
9025-53-0
Molecular Weight
mol wt 81 kDa
Purity
> 20 mg protein/mL (UV) > 90% (SDS-PAGE)
Unit Definition
One xylosidase unit will produce 1 μmole of o-nitrophenol per minute at 70°C from a 1mM solution of o-nitrophenyl-β-xyloside in 50 mM sodium acetate at pH 5.8.
Storage
2-8°C
Synonyms
β-Xylosidase; β-Xylosidase, thermostable; 9025-53-0
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