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3-Hydroxybutyrate dehydrogenase from Prokaryote, Recombinant

Cat No.
NATE-1099
Description
In enzymology, a 3-hydroxybutyrate dehydrogenase (EC 1.1.1.30) is an enzyme that catalyzes the chemical reaction: (R)-3-hydroxybutanoate + NAD+ ↔ acetoacetate + NADH + H+. Thus, the two substrates of this enzyme are (R)-3 hydroxybutanoate and NAD+, whereas its three products are acetoacetate, NADH, and H+. This enzyme belongs to the family of oxidoreductases, to be specific, those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor.
Abbr
3-HBDH, Recombinant (Prokaryote)
Alias
3-HBDH
Source
Prokaryote
Applications
High purity recombinant 3-Hydroxybutyrate dehydrogenase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 1.1.1.30
Activity
140 U/mg protein at pH 8.0 and 25°C.
CAS No.
9028-38-0
Molecular Weight
~ 29,177
Isoelectric point
~ 6.9
Unit Definition
One Unit of 3-hydroxybutyrate dehydrogenase (3-HBDH) is defined as the amount of enzyme required to produce one μmole of NADH from NAD+.
Optimum pH
8
Thermal stability
up to 25°C
Stability
> 2 years at 4°C
Storage
4°C
Preparation Instructions
For assay, this enzyme should be diluted in 200 mM Tris/HCl buffer, pH 8.0. Swirl to mix the enzyme suspension immediately prior to use.
Synonyms
3-hydroxybutyrate dehydrogenase; 3-HBDH; NAD-β-hydroxybutyrate dehydrogenase; hydroxybutyrate oxidoreductase; β-hydroxybutyrate dehydrogenase; D-β-hydroxybutyrate dehydrogenase; D-3-hydroxybutyrate dehydrogenase; D- (–)-3-hydroxybutyrate dehydrogenase; β-hydroxybutyric acid dehydrogenase; 3-D-hydroxybutyrate dehydrogenase; β-hydroxybutyric dehydrogenase; EC 1.1.1.30; 9028-38-0
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Inquiry
Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0003
EC No.EC 1.1.1.30
CAS No.9028-38-0
SourcePseudomonas lemoignei
CatalogNATE-0004
EC No.EC 1.1.1.30
CAS No.9028-38-0
SourceRhodopseudomonas sphaeroides
CatalogNATE-0005
EC No.EC 1.1.1.30
CAS No.9028-38-0
SourceAlcaligenes faecalis
Realted Service
Realted Protocols
ß-HYDROXYBUTYRATE DEHYDROGENASE -Enzymatic Assay Protocol
(S)-DIACETYL REDUCTASE -Enzymatic Assay Protocol
s-diacetyl reductase -Enzymatic Assay Protocol
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