Applications
4-Chloro-7-nitrobenzofurazan (NBD-chloride) is a fluorescence assay reagent used to label free sulfhydryls and N-terminals within proteins. NBD-chloride is used as an enzyme inhibitor and as a trapping agent for cysteine sulfenic acid.
Enzyme Commission Number
233-496-4
Synonyms
4-Chloro-7-nitro-1, 2, 3-benzoxadiazole; NBD-chloride
Molecular Formula
C6H2ClN3O3
PubChem Substance ID
24855392
Beilstein Registry Number
614212
Substrates
Glutathione S-Transferase
Impurities
λex 336 nm in methanol