Applications
ABTS Buffer is a solvent for ABTS. ABTS solution is the ideal substrate solution for enzyme immunoassays with horseradish peroxidase (HRP) as a marker enzyme. Because of the high sensitivity of the reaction, autoreaders will often display "over" after several minutes.
Preparation Instructions
Working solution: Dissolve the contents of one bottle substrate for ABTS in 100 ml double-distilled water. This concentrate is stable when stored in aliquots at -15 to -25 °C.To obtain the working solution, dilute one aliquot in a ratio of 1:10 with double-dist. water. Dissolve one ABTS tablet (50 mg) in 50 ml of the working solution. This solution has a light-green color. In microtiter plates the solution is colorless. A405 nm/1 cm should be < 0.16.Work as clean as possible. Heavy metal ions or traces of peroxidase can produce a dark green ABTS solution. Such solutions are not suitable for the assay.
Shipping Conditions
wet ice
Substrates
Peroxidase Substrates
