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Acetyl-CoA synthetase from Bacillus subtilis, Recombinant

Cat No.
NATE-0797
Description
Acetyl-CoA synthetase or Acetate-CoA ligase is an enzyme (EC 6.2.1.1) involved in metabolism of acetate. It is in the ligase class of enzymes, meaning that it catalyzes the formation of a new chemical bond between two large molecules.
Abbr
Acetyl-CoA synthetase, Recombinant (B. subtilis)
Source
Bacillus subtilis
Applications
High purity Acetyl-CoA synthetase (B. subtilis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
In 3.2 M ammonium sulphate
Enzyme Commission Number
EC 6.2.1.1
Activity
39 U/mg protein at pH 8.4 and 37°C; 7.6 U/mg protein at pH 7.5 and 37°C.
CAS No.
9012-31-1
Molecular Weight
~ 65,975 Da
Isoelectric point
~ 5.8
Unit Definition
One Unit of acetyl-CoA synthetase is defined as the amount of enzyme required to produce one μmole of NADH from NAD+.
Optimum pH
8.4
Optimum temperature
37°C
Stability
Stable at 4°C for > 2 years.
Storage
Store at 4°C.
Preparation Instructions
For assay, this enzyme should be diluted in 400 mM TEA buffer, pH 8.4 containing 0.5 mg/ml BSA, 30 mM L-Malic acid and 10 mM MgCl2. Swirl to mix the enzyme suspension immediately prior to use.
Synonyms
Acetyl-CoA synthetase; Acetate-CoA ligase; EC 6.2.1.1; Acetate thiokinase; Acetyl-activating enzyme; Acetyl-CoA synthase; Acyl-activating enzyme
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