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Acid phosphatase 1 from Human, recombinant

Cat No.
NATE-1672
Description
Acid phosphatase 1, also known as ACP1, belongs to the phosphotyrosine protein family. It functions as an acid phosphatase and a protein tyrosine phosphatase (PTPase) and is present in all human tissues, including adipocytes. This enzyme hydrolyzes protein tyrosine phosphate to protein tyrosine and orthophosphate, and also orthophosphoric monoesters to alcohol and orthophosphate.
Abbr
ACP1, Recombinant (Human)
Source
E. coli
Species
Human
Form
Liquid
Enzyme Commission Number
EC 3.1.3.2
Activity
> 60,000 unit/mg of protein
CAS No.
9001-77-8
Molecular Weight
20.1 kDa (178 aa, 1-158 aa + NT His-Tag)
Purity
> 95% by SDS-PAGE
Concentration
1 mg/ml
Unit Definition
One unit is defined as the amount of enzyme that will hydrolyze 1 nmole of p-nitrophenyl phosphate per minute at 37°C in MES pH 5.0 using 10 mM of substrate.
Storage
Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Synonyms
Acid phosphatase 1; soluble isoform b; ACP1; HAAP; LMW-PTP; Red cell acid phosphatase 1; Adipocyte acid phosphatase
Endotoxin Level
< 1.0 EU per 1 μg of protein (determined by LAL method)
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Realted Protocols
phosphatase,acid,prostatic -Enzymatic Assay Protocol
PHYTASE -Enzymatic Assay Protocol
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