Description
Acid phosphotase is one of the main enzymes of Mycobacterium tuberculosis metabolism. Acid phosphotase is a probable target for antituberculosis drugs.
Abbr
ACP, Recombinant (E. coli)
Applications
Acid phosphotase is one of the main enzymes of Mycobacterium tuberculosis metabolism. Acid phosphotase is a probable target for antituberculosis drugs.
Form
In 3.2 M ammonium sulphate containing 1 mM MgCl2 and 0.1 mM ZnSO4.
Enzyme Commission Number
EC 3.1.3.2
Activity
25 U/mg protein at pH 5.0 and 40°C
Molecular Weight
~ 24,667
Unit Definition
One Unit of acid phosphatase is defined as the amount of enzyme required to produce one µmole of p-nitrophenol from 4-nitrophenyl phosphate per minute at 40°C measured at 410 nm.
Stability
> 2 years at 4°C.
Preparation Instructions
For assay, this enzyme should be diluted in assay buffer containing 0.5 mg/mL BSA, 1 mM magnesium and 0.1 mM zinc. Swirl to mix the enzyme suspension immediately prior to use.
Synonyms
EC 3.1.3.2; acid phosphomonoesterase; phosphomonoesterase; glycerophosphatase; acid monophosphatase; acid phosphohydrolase; acid phosphomonoester hydrolase; uteroferrin; acid nucleoside diphosphate phosphatase; orthophosphoric-monoester phosphohydrolase (acid optimum)
