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Alanine Aminotransferase from Human, Recombinant

Cat No.
NATE-0802
Description
Alanine transaminase (ALT) is a transaminase enzyme. It is also called alanine aminotransferase (ALAT) and was formerly called serum glutamate-pyruvate transaminase (SGPT) or serum glutamic-pyruvic transaminase (SGPT). ALT is found in plasma and in various body tissues, but is most common in the liver. It catalyzes the two parts of the alanine cycle. Serum ALT level, serum AST (aspartate transaminase) level, and their ratio (AST/ALT ratio) are commonly measured clinically as biomarkers for liver health. The tests are part of blood panels.
Abbr
ALT, Recombinant (Human)
Alias
ALT; ALAT; GPT
Source
E. coli
Species
Human
Appearance
Sterile liquid formulation.
Product Overview
Alanine Aminotransferase Human Recombinant produced in E. coli is a homodimer, nonglycosylated, polypeptide chain containing 495a.a and having a molecular mass of 54,479 Dalton. The amino acid sequence is the same as that of native form of human liver ALT. The ALT is purified by proprietary chromatographic techniques.
Activity
1,000 U/mg
CAS No.
9000-86-6
Molecular Weight
54,479 Da
Purity
Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Stability
AAT1 although stable at 10°C for 5 days, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.
Buffer
The protein was dialyzed against 40mM sodium acetate buffer (pH 5.5), 1mM DTT,1mM EDTA, 5mM 2-oxoglutarate and 0.1mM pyridoxal-5'-phosphate.
Synonyms
ALT1; Glutamic-pyruvic transaminase 1; GPT 1; Glutamic-alanine transaminase 1; AAT1; ALT; ALAT; SGPT; Alanine transaminase; alanine aminotransferase; GPT; β-alanine aminotransferase; alanine-α-ketoglutarate aminotransferase; alanine-pyruvate aminotransferase; glutamic acid-pyruvic acid transaminase; glutamic-pyruvic aminotransferase; L-alanine aminotransferase; L-alanine transaminase; L-alanine-α-ketoglutarate aminotransferase; pyruvate transaminase; pyruvate-alanine aminotransferase; pyruvate-glutamate transaminase
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Inquiry
Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-1819
EC No.EC 2.6.1.21
CAS No.37277-85-3
SourceE. coli
CatalogNATE-0064
EC No.EC 2.6.1.2
CAS No.9000-86-6
SourceE. coli
CatalogNATE-0065
EC No.EC 2.6.1.2
CAS No.9000-86-6
SourceHuman Liver
CatalogNATE-0066
EC No.EC 2.6.1.2
CAS No.9000-86-6
SourceHuman Cardiac Tissue
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