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Alginate lyase from Sphingomonas sp., Recombinant

Cat No.
NATE-0805
Description
In enzymology, a poly (beta-D-mannuronate) lyase (EC 4.2.2.3) is an enzyme that catalyzes the chemical reaction:Eliminative cleavage of polysaccharides containing beta-D-mannuronate residues to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl groups at their ends. This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on polysaccharides. This enzyme participates in fructose and mannose metabolism.
Abbr
Alginate lyase, Recombinant (Sphingomonas sp.)
Alias
alginase I; alginase II
Source
Sphingomonas sp.
Applications
High purity recombinant Alginate lyase (Sphingomonas sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
In 3.2 M ammonium sulphate
Enzyme Commission Number
EC 4.2.2.3
Activity
125 U/mg protein (on sodium alginate) at pH 7.2 and 40°C.
CAS No.
9024-15-1
Molecular Weight
~ 39,600 Da
Isoelectric point
~ 5.6
pH Stability
4.0 - 9.0 (> 75% control activity after 24 hours at 4°C)
Specificity
Endo-acting β-elimination cleavage of the polysaccharide, alginate.
Unit Definition
One Unit of alginate lyase activity is defined as the amount of enzyme required to produce an increase in absorbance of 1.0 per minute at 235 nm and 40°C.
Optimum pH
7.2
Optimum temperature
40°C
Storage
Store at 4°C.
Buffer
0.02% (w/v) sodium azide
Preparation Instructions
For assay, this enzyme should be diluted in TRIS.HCl buffer (100 mM), pH 7.2 containing 1 mg/ml BSA. Swirl to mix the enzyme immediately prior to use.
Synonyms
alginate lyase I; alginate lyase; alginase I; alginase II; alginase; poly (β-D-1,4-mannuronide) lyase; EC 4.2.2.3
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