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Alkaline Phosphatase from E. coli, Recombinant

Cat No.
NATE-0806
Description
Alkaline phosphatase (ALP, ALKP, ALPase, Alk Phos) (EC 3.1.3.1) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase.
Abbr
ALP, Recombinant (E. coli)
Alias
ALP; ALKP
Source
E. coli
Applications
High purity recombinant Alkaline phosphatase (E. coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
In 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.1.3.1
Activity
10 U/mg protein at pH 10.4 and 37°C.
CAS No.
9001-78-9
Molecular Weight
~ 50,503 Da
Isoelectric point
~ 6.0
Unit Definition
One Unit of alkaline phosphatase is defined as the amount of enzyme required to produce one μmole of p-nitrophenol from 4-nitrophenyl phosphate per minute at 37°C measured at 410 nm.
Optimum pH
10.4
Optimum temperature
37°C
Stability
Stable at 4°C for > 2 years.
Storage
Store at 4°C.
Buffer
1 mM magnesium chloride and 0.1 mM zinc sulphate
Preparation Instructions
For assay, this enzyme should be diluted in assay buffer containing 1 mM magnesium and 0.1 mM zinc. Swirl to mix the enzyme suspension immediately prior to use.
Synonyms
Alkaline phosphatase; ALP; ALKP; ALPase; Alk Phos; EC 3.1.3.1; Alkaline phosphomonoesterase; Glycerophosphatase; Phosphomonoesterase
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