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apyrase

Cat No.
EXWM-4623
Description
Apyrases are active against both di- and triphosphate nucleotides (NDPs and NTPs) and hydrolyse NTPs to nucleotide monophosphates (NMPs) in two distinct successive phosphate-releasing steps, with NDPs as intermediates. They differ from ATPases, which specifically hydrolyse ATP, by hydrolysing both ATP and ADP. The eukaryotic enzymes requires Ca2+, but Mg2+ can substitute. Most of the ecto-ATPases that occur on the cell surface and hydrolyse extracellular nucleotides belong to this enzyme family.
Form
Liquid or lyophilized powder
Enzyme Commission Number
CAS No.
9000-95-7
Storage
Store it at +4 ºC for short term. For long term storage, store it at -20 ºC~-80 ºC.
Synonyms
ATP-diphosphatase; adenosine diphosphatase; ADPase; ATP diphosphohydrolase [ambiguous]
Reaction
a nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate (overall reaction); (1a) a nucleoside 5'-triphosphate + H2O = a nucleoside 5'-diphosphate + phosphate; (1b) a nucleoside 5'-diphosphate + H2O = a nucleoside 5'-phosphate + phosphate
Notes
This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
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Realted Protocols
APYRASE AMP as Substrate -Enzymatic Assay Protocol
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