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Arginine Specific Protease from Porphyromonas gingivalis
Cat No.
NATE-1602
Description
Arginine Specific Protease specifically digests peptides and proteins C-terminal to arginine residues. The protease is specific for Arg-X motifs and does not have activity at lysines as commonly observed using Arg-C. The enzymatic activity of Arginine Specific Protease includes digestion of Arg-Pro linkages, which is difficult to digest with other enzymes. The enzyme is active at pH ranging from pH 5.0-9.0 and in presence of denaturing agents such as 6M urea and 0.1% SDS.
Abbr
Arginine Specific Protease (Porphyromonas gingivalis)
Source
Porphyromonas gingivalis
Applications
Peptide mapping
Protein sequence analysis
Protein fingerprinting
Post-translational modification analysis
Protein sequence analysis
Protein fingerprinting
Post-translational modification analysis
Appearance
White to light yellow powder
Form
Lyophilized powder in 20 mM Bis-Tris, 150 mM NaCl, 5 mM CaCl2, Ph 6.8 without preservatives
Purity
> 95% homogeneity as determined by SDS-PAGE and HPLC.
pH Stability
5.0-9.0
Optimum pH
6.5-8.0
Stability
1 year when stored at -20°C. After reconstitution by addition of water it is stable for at least 1 month at 4-8°C.
Storage
The product is shipped on ice and should be stored at -20°C upon arrival.
Buffer
To maintain the enzymatically active cysteine in a reduced form, TCEP is added to preserve the reducing environment. Arginine Specific Protease is active in a broad pH range, 5.0-9.0, with optimal activity between pH 6.5-8.0. Digestion can be performed in the presence of chaotropic agents or detergents such as urea, SDS, tween, SDC. Buffers tested and compatible with Arginine Specific Protease activity are Tris, Bis-Tris and ammonium bicarbonate.
Synonyms
Arginine Specific Protease; GingisREX
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