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Biotinylated Transglutaminase from Human, Recombinant

Cat No.
NATE-1725
Description
This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with a phosphate group as acceptor.
Abbr
TG, Recombinant (Human)
Alias
TG
Source
Insect cells
Species
Human
Applications
Recombinant human transglutaminase is a homodimer (a2) composed of two chains held together by non covalent bonds. After activation of the zymogen by Thrombin and Ca2+ to its active form (a*2, Factor XIIIa), Factor XIIIa catalyzes the formation of covalent bridges (ε-(γ-glutamyl) lysine bonds) between fibrin units to increase the elasticity of the clot network. The resulting cross-linked fibrin is insoluble and resistant to lysis.
Appearance
Liquid
Package
50 μg
Form
The transglutaminase is formulated in 10 mM Sodium Phosphate pH 8.0, 15 mM NaCl. Sample contains 50% glycerol. hFXIII is a Ca2+-dependent enzyme.
Enzyme Commission Number
EC 2.3.2.13
Activity
> 750 U/mg [Activity is determined by measuring the rate of fluorescence enhancement after transglutaminase-catalyzed monodansylcadaverine-incorporation into N,N-dimethylated casein according to Lorand et al., Anal. Biochem. 44 (221-231).
CAS No.
80146-85-6
Molecular Weight
83 kDa
Purity
> 95 % (visually by SDS-PAGE)
Unit Definition
1 U is defined as the increase in fluorescence intensity of 1 a.u./min (measured on a Cary eclipse fluorescence spectrophotometer, Varian; λex = 332 nm, λem = 500 nm; band filter = 5 nm; detector strength = 600 V; temperature = 37°C, assay volume = 1 ml)].
Activators
Add Thrombin and 10 mM Ca2+ to activate transglutaminase.
Storage
Store working aliquots at ≤ - 20°C. Avoid repeated freezing and thawing.
Synonyms
transglutaminase; EC 2.3.2.13; 80146-85-6; transglutaminase; Factor XIIIa; fibrinoligase; fibrin stabilizing factor; glutaminylpeptide γ-glutamyltransferase; polyamine transglutaminase; tissue transglutaminase; R-glutaminyl-peptide:amine γ-glutamyl transferase; protein-glutamine γ-glutamyltransferase; TG1
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CatalogNATE-1723
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SourceE. coli
CatalogNATE-1724
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SourceInsect cells
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SourceInsect cells
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Realted Protocols
transglutaminase -Enzymatic Assay Protocol
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