Products

Enzymes for Research, Diagnostic and Industrial Use

Carbonic Anhydrase II from Bovine, Recombinant

Cat No.
NATE-1463
Description
The carbonic anhydrases (or carbonate dehydratases) form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs relatively slowly in the absence of a catalyst. The active site of most carbonic anhydrases contains a zinc ion; they are therefore classified as metalloenzymes.
Abbr
CA2, Recombinant (Bovine)
Alias
CA2; CA II
Source
E. coli
Species
Bovine
Applications
Carbonic anhydrase is used to create carbon dioxide capture systems and to research various purification techniques. Carbonic anhydrase is also used to study acid-base regulation in fish and carbonic anhydrase type II deficiency syndrome. Bovine carbonic anhydrase II (CA II), has been widely used as a model protein in the investigation of the protein folding process.
Appearance
Colorless clear liquid
Product Overview
Carbonic Anhydrase is a zinc-containing enzyme that catalyzes the reversible conversion of carbon dioxide to bicarbonate. One of its main physiological roles is to maintain the acid-base balance in blood and other tissues. Lack of carbonic anhydrase results in carbonic anhydrase type II deficiency syndrome, which is an autosomal recessive disease that causes osteopetrosis, renal tubular acidosis and brain calcifications. Carbonic anhydrases are metalloenzymes since most active sites contain zinc. Carbonic anhydrases are widely distributed in plant and animal tissues where they are involved in diverse physiological processes, such as photosynthesis, pH homeostasis, calcification, and bone resorption. The enzymes can be localized in the cytosol or mitochondria, be membrane bound with extracellular domains, or be secreted. Bovine carbonic anhydrase II (CA II) is a cytosolic, single-chain, approximately 29 kDa enzyme.
Form
Supplied as a solution in 20 mM Tris, pH 7.6, with 150 mM NaCl.
Enzyme Commission Number
EC 4.2.1.1
Activity
> 5,000 units/mg
CAS No.
9001-03-0
Molecular Weight
29-31 kDa
Purity
>90% by SDS-PAGE
Concentration
500-700 µg/ml
Unit Definition
One unit will decrease th pH of a 20 mM tris buffer from pH 8.3 to 6.3 per minute at 0 °C.
Stability
The product is stable for at least 2 years as supplied.
Storage
Store the product at –20 °C. After initial thawing, the enzyme should be refrozen at –20 °C in aliquots.
Synonyms
carbonic anhydrases; carbonate dehydratases; EC 4.2.1.1; anhydrase; carbonate anhydrase; carbonic acid anhydrase; carboxyanhydrase; carbonic anhydrase A; carbonate hydro-lyase
Download Datasheet:


“Carbonic Anhydrase” Total Products Page

Inquiry
Related Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-1669
EC No.EC 4.2.1.1
CAS No.
SourceE. coli
CatalogNATE-1678
EC No.EC 4.2.1.1
CAS No.9001-03-0
SourceHuman Liver
CatalogNATE-0097
EC No.EC 4.2.1.1
CAS No.9001-03-0
SourceHuman erythrocytes
CatalogNATE-0098
EC No.EC 4.2.1.1
CAS No.9001-03-0
SourceE. coli
+ See More >>
Related Protocols
enolase -Enzymatic Assay Protocol
crotonase -Enzymatic Assay Protocol
Online Inquiry
Name:
*Phone:
*E-mail Address:
Country or Region:
Company/Institution:
*Products or Services Interested:
Quantity:
Project Description:
Our Products are Not Intended for Private Therapeutic Use!
*Verification Code:
Please input "enzymes"(case insensitive) as verification code.

Welcome! For price inquiries, please feel free to contact us through the form on the left side. We will get back to you as soon as possible.


Sitemap  Privacy Policy  Terms and Conditions
Copyright ©2011 - 2018 Creative Enzymes.
Contact Us 45-1 Ramsey Road, Shirley, NY 11967, USA
Email: info@creative-enzymes.com
Tel: 1-631-562-8517 1-516-512-3133
Fax: 1-631-938-8127
Distributors To view the contact information for a specific location, select the desired country:
       
0
Click unfold / close
Inquiry Basket
Delete selected Check Out
Decide to move out of the shopping cart??

Sure No, Back
 

Please choose product!

< Go Back
You have already added to buy this product