Description
Ces1d, also known as carboxylesterase 1D, is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds. It is the principle lipase of white adipose tissue fat cake extracts. Partially purified white adipose tissue Ces1d had lipase activity as well as lesser but detectable neutral cholesteryl ester hydrolase activity. The protein shows low catalytic efficiency for hydrolysis of CPT-11, a prodrugs for camptothecin used in cancer therapeutics. Recombinant mouse Ces1d, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Abbr
CES1D, Recombinant (Mouse)
Source
Insect cell (Baculovirus) and fused to His-tag at N-terminus
Activity
> 80,000 pmol/min/ug
Molecular Weight
60.9 kDa
Unit Definition
Defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to pnitrophenol per minute at pH 7.5 at 37°C.
Storage
Store at +4°C for short term (1-2 weeks). For long term storage, aliquot and store at -70°C. Avoid repeated freeze/thaw cycles.
Synonyms
Ces1d; Carboxylesterase 3; FAEE synthase; TGH; Ces3
Endotoxin Level
< 1 EU/μg
Formulation
Sterile Filtered lyophilized (freeze-dried) powder from 50 mM Tris, 100 mM NaCl, pH 8.0.
