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Cholesterol Esterase from E. coli, Recombinant

Cat No.
DIA-405
Description
Sterol esterase belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name of this enzyme class is steryl-ester acylhydrolase. This enzyme participates in bile acid biosynthesis.
Abbr
CE, Recombinant (E. coli)
Source
E. coli
Species
E. coli
Appearance
Light yellow lyophilizate
Enzyme Commission Number
EC 3.1.1.13
Activity
> 5 U/mg lyophilizate
CAS No.
9026-00-0
Molecular Weight
ca. 54 kDa
pH Stability
5.0-10.0
Michaelis Constant
1.9 x 10^-5 M (cholesterol linoleate)
Structure
monomer of 54 kDa (SDS-PAGE)
Specificity
cholesterol linoleate (100), cholesterol acetate (2), cholesterol oleate (98), cholesterol palmitate (74), cholesterol stearate (68), cholesterol arachidonate (46)
Unit Definition
One unit (U) is defined as the amount of enzyme which produces 1 μmol of cholesterol per min at 37°C and pH 6.0.
Optimum pH
5.5–7.0
Optimum temperature
40°C
Thermal stability
below 50°C
Stabilizers
Sucrose
Synonyms
cholesterol esterase; cholesteryl ester synthase; triterpenol esterase; cholesteryl esterase; cholesteryl ester hydrolase; sterol ester hydrolase; cholesterol ester hydrolase; cholesterase; acylcholesterol lipase; EC 3.1.1.13; Sterol esterase
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Related Protocols
CHOLESTEROL ESTERASE -Enzymatic Assay Protocol
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