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Citrate synthase from E. coli, Recombinant

Cat No.
NATE-1059
Description
Citrate synthase catalyses the conversion of Citrate to acetyl-CoA in the presence of coenzyme-A with the release of H2O and oxaloacetate. The enzyme has a molecular weight of 85 kDa and a pI of 6.1-6.6. It is inhibited by fluoroacetyl-CoA, palmitoyl-CoA, and citroyl-CoA. It is also inhibited when it is acetylated by acetic anhydride or iodinated by iodine.
Abbr
CS, Recombinant (E. coli)
Alias
CS
Source
E. coli
Applications
High purity recombinant Citrate synthase (E. coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
In 3.2 M ammonium sulphate
Enzyme Commission Number
EC 2.3.3.1 (Modified from 4.1.3.7 in 2002)
Activity
14 U/mg protein at pH 8.0 and 25°C.
CAS No.
9027-96-7
Molecular Weight
~ 50,178
Isoelectric point
~ 6.5
Unit Definition
One Unit of Citrate synthase is defined as the amount of enzyme required to produce one μmole of citric acid from oxaloacetic acid and acetyl-CoA measured at 232 nm.
Optimum pH
8
Optimum temperature
25°C
Stability
> 2 years at 4°C.
Storage
4°C
Preparation Instructions
For assay, this enzyme should be diluted in 100 mM imidazole buffer, pH 8.0. Swirl to mix the enzyme suspension immediately prior to use.
Synonyms
CS; EC 4.1.3.7; EC 2.3.3.1; 9027-96-7; Citrate (Si)-synthase; (R)-citric synthase; Citrate oxaloacetate-lyase [(pro-3S)-CH2COO-->acetyl-CoA]; acetyl-CoA:oxaloacetate C-acetyltransferase; [thioester-hydrolysing, (pro-S)-carboxymethyl forming]
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