Description
Collagenase AF-1 GMP Grade, often in combination with Neutral Protease AF GMP Grade, is suitable for the dissociation of sensitive cells from several tissues, such as pancreas, liver and dental pulp. It has been shown to be highly effective for the isolation of islets of Langerhans from human pancreas intended for transplantation.
Source
Clostridium histolyticum
Applications
Collagenase AF-1 GMP Grade is suitable for cell isolation from several tissue types intended for clinical applications. It is often used in combination with Neutral Protease AF GMP Grade.
Enzyme Commission Number
EC 3.4.24.3
Unit Definition
1 U according to Wünsch catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.
Synonyms
Collagenase AF-1; Collagenase; Collagenase AF-1 GMP; Collagenase GMP
Endotoxin Level
≤ 10.0 EU/mg
