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Creatinase from E. coli, Recombinant

Cat No.
NATE-1241
Description
In enzymology, a creatinase (EC 3.5.3.3) is an enzyme that catalyzes the chemical reaction:creatine + H2O↔ sarcosine + urea. Thus, the two substrates of this enzyme are creatine and H2O, whereas its two products are sarcosine and urea. This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amidines. Creatinase accelerates the conversion reaction of creatine and water molecule to sarcosine and urea. It always acts in homodimer state and is induced by choline chloride.
Abbr
Creatinase, Recombinant (E. coli)
Source
E. coli
Species
E. coli
Appearance
White lyophilizate
Enzyme Commission Number
EC 3.5.3.3
Activity
> 9 U/mg lyophilizate
CAS No.
37340-58-2
Contaminants
catalase < 0.5%
Molecular Weight
ca. 80 kDa
pH Stability
4.0–11.0
Michaelis Constant
8.6 x 10^-3 M (creatine)
Structure
2 subunits of 48 kDa (SDS-PAGE)
Unit Definition
One unit (U) is defined as the amount of enzyme which produces 1 μmol of urea per min at 37°C and pH 7.7.
Optimum pH
7.0–9.0
Optimum temperature
45°C
Thermal stability
below 53°C
Storage
at -20°C
Stabilizers
Sucrose
Inhibitors
Hg2+
Synonyms
Creatine amidohydrolase; Creatinase; EC 3.5.3.3
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogDIA-141
EC No.EC 3.5.3.3
CAS No.37340-58-2
SourceMicroorganism
CatalogDIA-185
EC No.EC 3.5.3.3
CAS No.37340-58-2
SourceMicroorganism
CatalogNATE-0160
EC No.EC 3.5.3.3
CAS No.37340-58-2
SourceActinobacillus sp.
CatalogNATE-0161
EC No.EC 3.5.3.3
CAS No.37340-58-2
SourceFlavobacterium sp.
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Realted Protocols
CREATINASE -Enzymatic Assay Protocol
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