Creatinase from E. coli, Recombinant

Cat No.

NATE-1241

Description

In enzymology, a creatinase (EC 3.5.3.3) is an enzyme that catalyzes the chemical reaction:creatine + H2O↔ sarcosine + urea. Thus, the two substrates of this enzyme are creatine and H2O, whereas its two products are sarcosine and urea. This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amidines. Creatinase accelerates the conversion reaction of creatine and water molecule to sarcosine and urea. It always acts in homodimer state and is induced by choline chloride.

Abbr

Creatinase, Recombinant (E. coli)

Source

E. coli

Species

E. coli

Appearance

White lyophilizate

Enzyme Commission Number

EC 3.5.3.3

Activity

> 9 U/mg lyophilizate

CAS No.

37340-58-2

Contaminants

catalase < 0.5%

Molecular Weight

ca. 80 kDa

pH Stability

4.0–11.0

Michaelis Constant

8.6 x 10^-3 M (creatine)

Structure

2 subunits of 48 kDa (SDS-PAGE)

Unit Definition

One unit (U) is defined as the amount of enzyme which produces 1 μmol of urea per min at 37°C and pH 7.7.

Optimum pH

7.0–9.0

Optimum temperature

45°C

Thermal stability

below 53°C

Storage

at -20°C

Stabilizers

Sucrose

Inhibitors

Hg2+

Synonyms

Creatine amidohydrolase; Creatinase; EC 3.5.3.3

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