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Cytidylate kinase from Prokaryote, Recombinant

Cat No.
NATE-0824
Description
(d)CMP kinase (EC 2.7.4.25, prokaryotic cytidylate kinase, deoxycytidylate kinase, dCMP kinase, deoxycytidine monophosphokinase) is an enzyme with system name ATP:(d)CMP phosphotransferase. This enzyme catalyses the following chemical reaction: ATP + (d)CMP ↔ ADP + (d)CDP. The prokaryotic cytidine monophosphate kinase specifically phosphorylates CMP (or dCMP).
Abbr
dCMP kinase, Recombinant (Prokaryote)
Source
Prokaryote
Applications
High purity recombinant Cytidylate kinase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
In 3.2 M ammonium sulphate
Enzyme Commission Number
EC 2.7.4.25
Activity
17 U/mg protein at pH 7.6 and 25°C.
Molecular Weight
~ 26,910 Da
Isoelectric point
~ 6.2
Unit Definition
One Unit of cytidylate kinase is defined as the amount of enzyme required to produce one μmole of NAD+ from NADH.
Optimum pH
7.6
Optimum temperature
25°C
Stability
> 2 years at 4°C.
Storage
Store at 4°C.
Preparation Instructions
For assay, this enzyme should be diluted in 100 mM TEA buffer, pH 7.6 containing 1 mg/ml BSA. Swirl to mix the enzyme suspension immediately prior to use.
Synonyms
Cytidylate kinase; (d)CMP kinase; EC 2.7.4.25; prokaryotic cytidylate kinase; deoxycytidylate kinase; dCMP kinase; deoxycytidine monophosphokinase
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ProductName
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Source
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CatalogNATE-1828
EC No.EC 2.7.4.25
CAS No.
SourceE. coli
CatalogEXWM-3207
ProductName(d)CMP kinase
EC No.EC 2.7.4.25
CAS No.
Source
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