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Cytosolic 5'-nucleotidase II from Huamn, Recombinant


Cat No.
NATE-1742
Description
E.coli
Abbr
cN-II, Recombinant (Human)
Alias
cN-II
Source
Human
Species
cN-II
Applications
Human cytosolic IMP/GMP specific 5'-nucleotidase/phosphotransferase II (cN-II) is a pure and active protein of 65kDa cloned by RT-PCR amplification of mRNA extracted from human hepatoma cells and expressed in E.coli. The sequence of the cloned NT5C2 gene (GenBank accession number P49902) was confirmed by DNA sequencing (100% identity).
Package
stable lyophilized form
Enzyme Commission Number
EC 3.1.3.5
Activity
≥ 0.150 unit/mg protein
CAS No.
9027-73-0
Molecular Weight
65kDa
Unit Definition
One unit of 5’nucleotidase converts 1.0 µmole of IMP to inosine per minute at pH 7.6 at 37°C, as measured by a coupled PNP/XDH enzyme system in the presence of 20mM MgCl2, 5mM DTT, 500µM KH2PO4, and 1.25mM IMP.
Storage
–20 °C in a solution containing 50 mM Tris-HCl, pH 7.6, 2 mM β-mercaptoethanol, 50% glycerol.
Synonyms
uridine 5'-nucleotidase; 5'-adenylic phosphatase; adenosine 5'-phosphatase; AMP phosphatase; adenosine monophosphatase; 5'-mononucleotidase; AMPase; UMPase; snake venom 5'-nucleotidase; thimidine monophosphate nucleotidase; 5'-AMPase; 5'-AMP nucleotidase; AMP phosphohydrolase; IMP 5'-nucleotidase; EC 3.1.3.5; CD73; NT5E; ecto-5'-nucleotidase
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