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Endo-β-galactosidase from Bacteroides fragilis, Recombinant

Cat No.
NATE-1413
Description
Endo-β-Galactosidase is an enzyme that hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles the Escherichia freundii enzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide.
Abbr
Endo-β-galactosidase, Recombinant (Bacteroides fragilis)
Source
E. coli
Species
Bacteroides fragilis
Form
Sterile-filtered in 20 mM Tris-HCl, pH 7.5.
Enzyme Commission Number
EC 3.2.1.103
Activity
>14 U/ml; Specific Activity: >140 U/mg protein
CAS No.
55072-01-0
Molecular Weight
~32 kDa
Unit Definition
One unit is defined as the amount of enzyme that will liberate one µmol reducing sugar from bovine corneal keratan sulfate per min at 37°C, pH 5.8.
Storage
2-8°C
Synonyms
β-Galactosidase bacterial; Keratanase; Endo-β-galactosidase; keratan sulfate endogalactosidase; keratan-sulfate 1,4-β-D-galactanohydrolase; EC 3.2.1.103
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Related Protocols
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