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endo-1,3-β-D-Glucanase from Barley, Recombinant

Cat No.
NATE-1080
Description
Glucan endo-1,3-beta-D-glucosidase is an enzyme with system name 3-beta-D-glucan glucanohydrolase. This enzyme catalyses the following chemical reaction:Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans. This enzyme is marginally active on mixed-link (1->3,1->4)-beta-D-glucans.
Abbr
endo-1,3-β-D-Glucanase, Recombinant (Barley)
Source
Barley
Applications
High purity recombinant endo-1,3-beta-Glucanase (Barley) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
A solution containing 50% glycerol plus 0.02% (w/v) sodium azide.
Enzyme Commission Number
EC 3.2.1.39
Activity
154 U/mg protein at pH 5.0 and 40°C.
CAS No.
9025-37-0
Molecular Weight
~ 34,100
Isoelectric point
~ 8.4
pH Stability
3.0-9.0
Unit Definition
One Unit of β-1,3-glucanase activity is defined as the amount of enzyme required to release one µmole of glucose reducing-sugar equivalents per minute from laminarin (10 mg/mL) in sodium acetate buffer (100 mM) pH 5.0.
Optimum pH
5
Optimum temperature
40-50°C
Thermal stability
up to 50°C
Storage
Stored at -20°C. For assay, this enzyme should be diluted in sodium acetate buffer (100 mM), pH 5.0 containing 1 mg/mL BSA.
Synonyms
endo-1,3-β-glucanase; laminarinase; laminaranase; oligo-1,3-glucosidase; endo-1,3-β-glucanase; callase; β-1,3-glucanase; kitalase; 1,3-β-D-glucan 3-glucanohydrolase; endo- (1,3)-β-D-glucanase; (1→3)-β-glucan 3-glucanohydrolase; endo-1,3-β-D-glucanase; endo-1,3-β-glucosidase; 1,3-β-D-glucan glucanohydrolase; EC 3.2.1.39; 9044-93-3
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