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endo-1,4-β-Galactanase from Aspergillus niger, Recombinant

Cat No.
NATE-0829
Description
Arabinogalactan endo-beta-1,4-galactanase is an enzyme with system name arabinogalactan 4-beta-D-galactanohydrolase. The enzyme specifically hydrolyses (1->4)-beta-D-galactosidic linkages in type I arabinogalactans. This enzyme is isolated from the bacterium Bacillus subtilis.
Abbr
endo-1,4-β-Galactanase, Recombinant (Aspergillus niger)
Source
Aspergillus niger
Applications
High purity endo-1,4-β-Galactanase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
In 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.2.1.89
Activity
~ 400 U/mg at 40°C and pH 4.0.
CAS No.
58182-40-4
Molecular Weight
48 kDa
pH Stability
3.0-7.0 (at 4°C)
Specificity
Endohydrolysis of (1,4)-β-D-galactose linkages in (1,4)-β-galactans and type I arabinogalactans.
Unit Definition
One Unit of activity is defined as the amount of enzyme required to release one micromole of reducing-sugar equivalents from the substrate employed at pH 4 and 40°C.
Optimum pH
4.0-4.5
Optimum temperature
50°C
Stability
> 4 years at 4°C.
Storage
The enzyme is supplied as an ammonium sulphate suspension is the presence of 0.02% sodium azide. On dilution in buffer, the enzyme should be stored in the frozen state between use.
Synonyms
EC 3.2.1.89; endo-1,4-beta-galactanase; galactanase; arabinogalactanase; ganB (gene); Arabinogalactan endo-beta-1,4-galactanase; arabinogalactan 4-beta-D-galactanohydrolase; endo-1,4-β-Galactanase
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