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endo-1,4-β-Mannanase from Bacillus circulans, Recombinant

Cat No.
NATE-1111
Description
Mannan endo-1,4-beta-mannosidase is an enzyme with system name 4-beta-D-mannan mannanohydrolase. This enzyme catalyses the following chemical reaction: Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Abbr
endo-1,4 β-Mannanase, Recombinant (Bacillus circulans)
Source
Bacillus circulans
Applications
Applications established in the paper and pulp, food and feeds, oil extraction, coffee extraction, and detergent industries.
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.78
Activity
49 U/mg
CAS No.
37288-54-3
Molecular Weight
~ 52,000
Isoelectric point
~ 5.9
pH Stability
7.0 - 10.0
Specificity
Random hydrolysis of (1,4)-β-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Unit Definition
One Unit of mannanase activity is defined as the amount of enzyme required to release one µmole of mannose reducing-sugar equivalents per minute from carob galactomannan (5 mg/mL) in sodium phosphate buffer (100 mM), pH 7.5 at 40°C.
Optimum pH
7.0 - 9.0
Optimum temperature
60°C
Thermal stability
up to 50°C
Stability
> 2 years at 4°C
Storage
4°C
Preparation Instructions
For assay, this enzyme should be diluted in sodium phosphate buffer (100 mM), pH 7.5 containing 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Synonyms
endo-1,4-β-mannanase; endo-β-1,4-mannase; β-mannanase B; β-1, 4-mannan 4-mannanohydrolase; endo-β-mannanase; β-D-mannanase; mannan endo-1,4-β-mannosidase; 1,4-β-D-mannan mannanohydrolase; EC 3.2.1.78
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