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endo-1,4-β-Xylanase from Cellvibrio japonicus, Recombinant

Cat No.
NATE-1129
Description
Xylanase is the name given to a class of enzymes which degrade the linear polysaccharide beta-1,4-xylan into xylose, thus breaking down hemicellulose, one of the major components of plant cell walls. As such, it plays a major role in micro-organisms thriving on plant sources for the degradation of plant matter into usable nutrients. Xylanases are produced by fungi, bacteria, yeast, marine algae, protozoans, snails, crustaceans, insect, seeds, etc., (mammals do not produce xylanases).
Abbr
endo-1,4-β-Xylanase, Recombinant (Cellvibrio japonicus)
Source
Cellvibrio japonicus
Applications
High purity recombinant endo-1,4-beta-Xylanase (Cellvibrio japonicus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.8
Activity
38 U/mg protein (on wheat arabinoxylan) at pH 5.0 and 40°C; 62 U/mg protein (on wheat arabinoxylan) at pH 5.0 and 60°C.
CAS No.
9025-57-4
Molecular Weight
~ 39,000
Isoelectric point
~ 5.4
pH Stability
4.0 - 10.0
Unit Definition
One Unit of xylanase activity is defined as the amount of enzyme required to release one µmole of xylose reducing-sugar equivalents per minute from wheat arabinoxylan (5 mg/mL) in sodium acetate buffer (100 mM) pH 5.0.
Optimum pH
5
Optimum temperature
60°C
Thermal stability
up to 40°C
Storage
4°C
Preparation Instructions
For assay, this enzyme should be diluted in sodium acetate buffer (100 mM), pH 5.0 containing 0.5 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Synonyms
EC 3.2.1.8; endo- (1→4)-β-xylan 4-xylanohydrolase; endo-1,4-xylanase; xylanase; β-1,4-xylanase; endo-1,4-xylanase; endo-β-1,4-xylanase; endo-1,4-β-D-xylanase; 1,4-β-xylan xylanohydrolase; β-xylanase; β-1,4-xylan xylanohydrolase; endo-1,4-β-xylanase; β-D-xylanase; endo-1,4-β-xylanase
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