Description
Xylanase is the name given to a class of enzymes which degrade the linear polysaccharide beta-1,4-xylan into xylose, thus breaking down hemicellulose, one of the major components of plant cell walls. As such, it plays a major role in micro-organisms thriving on plant sources for the degradation of plant matter into usable nutrients. Xylanases are produced by fungi, bacteria, yeast, marine algae, protozoans, snails, crustaceans, insect, seeds, etc., (mammals do not produce xylanases).
Abbr
endo-1,4-β-Xylanase, Recombinant (Neocallimastix patriciarum)
Source
Neocallimastix patriciarum
Applications
High purity recombinant endo-1,4-beta-Xylanase (Neocallimastix patriciarum) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.8
Activity
1094 U/mg protein (on wheat arabinoxylan) at pH 6.0 and 40°C; 1497 U/mg protein (on wheat arabinoxylan) at pH 6.0 and 50°C.
Molecular Weight
~ 25,800
Unit Definition
One Unit of xylanase activity is defined as the amount of enzyme required to release one µmole of xylose reducing-sugar equivalents per minute from wheat arabinoxylan (5 mg/mL) in sodium phosphate buffer (100 mM) pH 6.0.
Thermal stability
up to 50°C
Preparation Instructions
For assay, this enzyme should be diluted in sodium phosphate buffer (100 mM), pH 6.0 containing 0.5 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Synonyms
EC 3.2.1.8; endo- (1→4)-β-xylan 4-xylanohydrolase; endo-1,4-xylanase; xylanase; β-1,4-xylanase; endo-1,4-xylanase; endo-β-1,4-xylanase; endo-1,4-β-D-xylanase; 1,4-β-xylan xylanohydrolase; β-xylanase; β-1,4-xylan xylanohydrolase; endo-1,4-β-xylanase; β-D-xylanase; endo-1,4-β-xylanase
