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Enzymes for Research, Diagnostic and Industrial Use

endo-1,4-β-Xylanase M4 from Aspergillus niger, Recombinant

Cat No.
NATE-0832
Description
Xylanase is the name given to a class of enzymes which degrade the linear polysaccharide beta-1,4-xylan into xylose, thus breaking down hemicellulose, one of the major components of plant cell walls. As such, it plays a major role in micro-organisms thriving on plant sources for the degradation of plant matter into usable nutrients. Xylanases are produced by fungi, bacteria, yeast, marine algae, protozoans, snails, crustaceans, insect, seeds, etc., (mammals do not produce xylanases).
Abbr
Xylanase 4, Recombinant (Aspergillus niger)
Alias
Xylanase
Source
Aspergillus niger
Applications
High purity endo-1,4-beta-Xylanase M4 (A. niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
In 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.2.1.8
Activity
79.3 U/mg (40°C, pH 4.5, wheat flour arabinoxylan as substrate).
CAS No.
9025-57-4
Molecular Weight
25 kDa
Isoelectric point
~ 3.7
pH Stability
3.0-8.0
Optimum pH
4.5
Optimum temperature
60°C
Stability
> 4 years at 4°C.
Storage
Store at 4°C.
Buffer
0.02% (w/v) sodium azide
Preparation Instructions
For the assay, enzyme preparation is diluted in 0.1M sodium acetate buffer pH 4.5 containing BSA (0.5 mg/mL). If BSA is excluded from the buffer lower activities are obtained.
Synonyms
EC 3.2.1.8; endo-(1→4)-β-xylan 4-xylanohydrolase; endo-1,4-xylanase; xylanase; β-1,4-xylanase; endo-1,4-xylanase; endo-β-1,4-xylanase; endo-1,4-β-D-xylanase; 1,4-β-xylan xylanohydrolase; β-xylanase; β-1,4-xylan xylanohydrolase; endo-1,4-β-xylanase; β-D-xylanase; endo-1,4-β-xylanase
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