Description
Xylanase is the name given to a class of enzymes which degrade the linear polysaccharide beta-1,4-xylan into xylose, thus breaking down hemicellulose, one of the major components of plant cell walls. As such, it plays a major role in micro-organisms thriving on plant sources for the degradation of plant matter into usable nutrients. Xylanases are produced by fungi, bacteria, yeast, marine algae, protozoans, snails, crustaceans, insect, seeds, etc., (mammals do not produce xylanases).
Abbr
Xylanase 6, Recombinant (Rumen microorganism)
Source
Rumen microorganism
Applications
High purity endo-1,4-beta-Xylanase M6 (rumen microorganism) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
In 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.2.1.8
Activity
380 U/mg (40°C, pH 6.0, wheat flour arabinoxylan as substrate).
Molecular Weight
26.5 kDa
pH Stability
6.0-8.5 (very unstable below pH 5.5)
Stability
> 4 years at 4°C.
Buffer
0.02% (w/v) sodium azide
Preparation Instructions
For the assay, enzyme preparation is diluted in 0.1M sodium acetate buffer pH 6.0 containing BSA (0.5 mg/mL). If BSA is excluded from the buffer lower activities are obtained.
Synonyms
EC 3.2.1.8; endo-(1→4)-β-xylan 4-xylanohydrolase; endo-1,4-xylanase; xylanase; β-1,4-xylanase; endo-1,4-xylanase; endo-β-1,4-xylanase; endo-1,4-β-D-xylanase; 1,4-β-xylan xylanohydrolase; β-xylanase; β-1,4-xylan xylanohydrolase; endo-1,4-β-xylanase; β-D-xylanase; endo-1,4-β-xylanase
