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Endoglycosidase S2 (Low Endotoxin) from Streptococcus pyogenes, Recombinant

Cat No.
NATE-1781
Description
EndoS2 LE is an endoglycosidase for deglycosylation of the Fc N-glycan moieties of IgGs (1). All IgG glycoforms are hydrolyzed, including high-mannose, hybrid-type and bisected glycans. EndoS2 LE hydrolyzes the β1,4 linkage between the core GlcNAc residues in the Fc-glycan, leaving the innermost GlcNAc on the Fc. EndoS2 LE deglycosylates all human IgG subclasses and IgG from the following species: mouse, rat, monkey, sheep, goat, cow and horse. It has also been reported to hydrolyze glycan moieties from alpha-1-acid glycoprotein. EndoS2 LE is a low endotoxin product, use endotoxin free materials and solutions.
Physiological reaction conditions at pH 7.4 and 37°C yields optimal enzyme activity. Other buffers and pH may be compatible but the reaction conditions needs to be tested to ensure efficient deglycosylation. The enzyme contains a His-tag and the molecular weight is 92 kDa.
Abbr
Endo S2, Recombinant (Streptocoocus pyogenes)
Source
E. coli
Species
Streptocoocus pyogenes
Form
Supplied lyophilized in 10 mM Tris, 150 mM NaCl, pH 7.6, with no preservatives added.
Unit Definition
One unit deglycosylates > 95% of 1 μg human IgG when incubated in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4 at 37°C for 30 min.
Storage
It is shipped at ambient temperature. It should be stored at -20°C upon arrival. After reconstitution EndoS2 LE is stable for 1 month at +4-8°C.
Synonyms
GlycINATOR LE; EndoS2; EndoS2 LE
Endotoxin Level
< 0.2 EU per vial
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CatalogNATE-1604
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CAS No.
SourceE.coli
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