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exo-Polygalacturonase from Yersinia enterocolitica, Recombinant

Cat No.
NATE-1645
Description
High purity recombinant exo-polygalacturonase (Yersinia enterocolitica) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Abbr
exo-Polygalacturonase, Recombinant (Yersinia enterocolitica)
Species
Yersinia enterocolitica
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.82
Activity
220 U/mg protein, 900 U/mL
CAS No.
37288-58-7
Molecular Weight
64 kDa
Isoelectric point
6.5
pH Stability
4.0-9.0 (> 75% control activity after 24 h at 4°C)
Specificity
Hydrolysis of polygalacturonic acid from the non-reducing end, releasing digalacturonate.
Unit Definition
One Unit of exo-polygalacturonase activity is defined as the amount of enzyme required to release one μmole of reducing-sugar equivalents per minute from polygalacturonic acid (10 mg/mL) in potassium phosphate buffer (100 mM) pH 6.0.
Optimum pH
6
Optimum temperature
60°C
Thermal stability
up to 50°C (diluted with 1 mg/mL BSA)
Stability
> 4 years at 4°C.
Preparation Instructions
For assay, this enzyme should be diluted in potasssium phosphate buffer (100 mM), pH 6.0 containing 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Synonyms
exo-poly-alpha-galacturonosidase
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Source
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