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Fructanase Mixture (purified-liquid)

Cat No.
NATE-1063
Description
This enzyme preparation is designed for use in the measurement of fructan (inulin) by the procedure of AOAC Method 997.08. The procedure recommends the use of Fructozyme, which is a fermentation product containing highly active exo-inulinase and endo-inulinase. However, Fructozyme also contains other enzymes at activity levels which interfere with the specific measurement of fructan or, alternatively, result in depolymerisation, and thus underestimation, of other dietary fibre components if this preparation is used in the standard AOAC dietary fibre methods to remove insoluble fructan.
Abbr
Fructanase Mixture (purified-liquid)
Product Overview
This product has been purified to remove α-galactosidase, β-glucanase and pectinase which interfere with the use of the preparation in the measurement of fructan, or in the solubilisation of "insoluble" fructan in the AOAC Total Dietary Fiber method.
Form
A stabilised solution containing 50% glycerol and 0.02% sodium azide.
Activity
exo-Inulinase 2,000 U/mL (on kestose at 40°C) endo-Inulinase ~ 100 U/mL (on fructan at 40°C) α-Galactosidase 0.09 U/mL (on p-nitrophenyl α-galactoside) β-Glucanase 0.05 U/mL (on β-glucan at 40°C) Pectinase < 0.30 U/mL (on pectin at 40°C).
Contaminants
α-galactosidase: 0.005% β-glucanase: 0.006% Pectinase: 0.02%
Stability
> 4 years at -20°C.
Storage
Stored at -20°C
Preparation Instructions
NOTE 1: This product has been purified to remove most of the α-galactosidase, β-glucanase and pectinase which interfere with the use of the preparation in the measurement of fructan, or in the solubilisation of “insoluble” fructan in the AOAC Total Dietary Fibre Methods. NOTE 2: For assay, enzyme preparation is diluted in 100 mM of sodium acetate buffer (pH 4.5) containing BSA (1 mg/mL). If BSA is excluded from the buffer, lower activities are obtained.
Synonyms
exo-Inulinase; endo-Inulinase; α-Galactosidase; β-Glucanase; Pectinase; Fructanase Mixture
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