Description
Geranylgeranyltransferase type 1 or simply geranylgeranyltransferase is one of the three enzymes in the prenyltransferase group. In specific terms, Geranylgeranyltransferase (GGTase 1) adds a 20-carbon isoprenoid called a geranylgeranyl group to proteins bearing a CaaX motif:a four-amino acid sequence at the carboxyl terminal of a protein. Geranylgeranyltransferase inhibitors are being investigated as anti-cancer agents.
Abbr
GGTase, Recombinant (Rat)
Source
baculovirus infected insect cells
Product Overview
>80% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells, > 200 units/mg protein
Activity
> 200 units/mg protein
Molecular Weight
mol wt 43 kDa (β subunit); mol wt 48 kDa (α subunit)
Unit Definition
One unit will transfer one nanomole of geranylgeranyl isoprenoid to H-Ras (CVLL isoform) per hour at pH 8.0 at 37°C.
Buffer
Solution in 50 mM Tris, pH 7.5, 1 mM DTT, and 0.2 mM octylglucoside.
Preparation Instructions
Host cells were coinfected with the α subunit of farnesyl transferase and the β subunit of geranylgeranyltransferase I.
Function
Contributes_to CAAX-protein geranylgeranyltransferase activity; CAAX-protein geranylgeranyltransferase activity; Contributes_to drug binding; Contributes_to isoprenoid binding; metal ion binding; Contributes_to peptide binding; prenyltransferase activity; protein binding; transferase activity; Contributes_to zinc ion binding
Synonyms
geranylgeranyltransferase type I; GGTase-I; GGTase; 160141-09-3; Geranylgeranyltransferase
