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Glucan 1,4-α-maltohexaosidase from Bacillus species, Recombinant

Cat No.
NATE-1644
Description
Glucan 1,4-alpha-maltohexaosidase (EC 3.2.1.98, exo-maltohexaohydrolase, 1,4-alpha-D-glucan maltohexaohydrolase) is an enzyme with systematic name 4-alpha-D-glucan maltohexaohydrolase. This enzyme catalyses the following chemical reaction: Hydrolysis of (1->4)-alpha-D-glucosidic linkages in amylaceous polysaccharides, to remove successive maltohexaose residues from the non-reducing chain ends. The products have the alpha-configuration.
Abbr
Glucan 1,4-α-maltohexaosidase, Recombinant (Bacillus species)
Species
Bacillus species
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.98
Activity
~ 800 U/mg protein (on soluble starch) at pH 8.5 and 40°C.
CAS No.
72561-12-7
Molecular Weight
56 kDa
Concentration
500 U/mL
Isoelectric point
6.5
pH Stability
6.0-9.0 (> 75% control activity after 24 h at 4°C)
Specificity
Hydrolysis of α-(1,4)-D-glucosidic linkages in amylaceous polysacchardies with predominant release of maltohexaose from short chain amylose (DP = 17).
Unit Definition
One Unit of glucan 1,4-α-maltohexaosidase activity is defined as the amount of enzyme required to release one μmole of maltose reducing sugar equivalents per minute from soluble starch (10 mg/mL) in sodium phosphate buffer (100 mM), pH 8.5 at 40°C.
Optimum pH
8.5
Optimum temperature
40°C
Thermal stability
up to 40°C (diluted with 1 mg/mL BSA)
Stability
> 4 years at 4°C.
Preparation Instructions
For assay, this enzyme should be diluted in sodium phosphate buffer (100 mM), pH 8.5 containing 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Synonyms
exo-maltohexaohydrolase; 1,4-alpha-D-glucan maltohexaohydrolase; 4-α-D-glucan maltohexaohydrolase4-α-D-glucan maltohexaohydrolase; glucan 1,4-alpha-maltohexaosidase; 4-alpha-D-glucan maltohexaohydrolase
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ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-1296
EC No.EC 3.2.1.98
CAS No.72561-12-7
SourceE. coli
CatalogEXWM-3961
EC No.EC 3.2.1.98
CAS No.72561-12-7
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