Products

Enzymes for Research, Diagnostic and Industrial Use

Glucoamylase P from Hormoconis resinae, Recombinant

Cat No.
NATE-1079
Description
Glucan 1,4-alpha-glucosidase is an enzyme located on the brush border of the small intestine with system name 4-alpha-D-glucan glucohydrolase. This enzyme catalyses the following chemical reaction:Hydrolysis0 of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose. Most forms of the enzyme can rapidly hydrolyse 1,6-alpha-D-glucosidic bonds when the next bond in the sequence is 1,4.
Abbr
Amyloglucosidase, Recombinant (Hormoconis resinae)
Source
Hormoconis resinae
Applications
High purity recombinant Amyloglucosidase (Glucoamylase P - H. Resinae) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.3
Activity
159 U/mg protein (on soluble starch) at pH 4.5 and 60°C; 64 U/mg protein (on soluble starch) at pH 4.5 and 40°C.
CAS No.
9032-08-0
Molecular Weight
~ 65,400
Isoelectric point
~ 4.9
pH Stability
3.0 - 9.0
Specificity
Hydrolysis of terminal non-reducing α-1,4-D-glycosidic bonds in α-1,4-D-glucans with "debranching activity" (hydrolysis of α-1,6-D-glycosidic bonds) in substrates such as starch and pullulan.
Unit Definition
One Unit of glucoamylase activity is defined as the amount of enzyme required to release one μg of β-D-glucose reducing-sugar equivalents per minute from soluble starch(10 mg/mL) in sodium acetate buffer (100 mM) at pH 4.5.
Optimum pH
4.0 - 5.0
Optimum temperature
60°C
Thermal stability
up to 50°C
Stability
> 4 years at 4°C.
Storage
4°C
Preparation Instructions
For assay, this enzyme should be diluted in sodium acetate buffer (100 mM), pH 4.5 containing 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Synonyms
glucoamylase; amyloglucosidase; γ-amylase; lysosomal α-glucosidase; acid maltase; exo-1,4-α-glucosidase; glucose amylase; γ-1,4-glucan glucohydrolase; acid maltase; 1,4-α-D-glucan glucohydrolase; EC 3.2.1.3; 9032-08-0
Download Datasheet:


“Glucoamylase” Total Products Page

Inquiry
Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogDIA-190
EC No.EC 3.2.1.3
CAS No.9032-08-0
SourceRhizopus sp.
CatalogNATE-0075
EC No.EC 3.2.1.3
CAS No.9032-08-0
SourceAspergillus niger
CatalogNATE-0076
EC No.EC 3.2.1.3
CAS No.9032-08-0
SourceRhizopus sp.
CatalogNATE-1051
EC No.EC 3.2.1.3
CAS No.9032-08-0
SourceAspergillus niger
+ See More >>
Realted Service
Realted Protocols
ß-N-ACETYLGLUCOSAMINIDASE -Enzymatic Assay Protocol
HYALURONIDASE -Enzymatic Assay Protocol
Online Inquiry
Name:
*Phone:
*E-mail Address:
Country:
Company/Institution:
*Products or Services Interested:
Quantity:
Project Description:
Our Products are Not Intended for Private Therapeutic Use!
*Verification Code:
Please input "enzymes"(case insensitive) as verification code.

Welcome! For price inquiries, please feel free to contact us through the form below through the form on the left side. We will get back to you as soon as possible.

Sitemap  Privacy Policy
Copyright ©2011 - 2017 Creative Enzymes.
Contact Us 45-1 Ramsey Road, Shirley, NY 11967, USA
Email: info@creative-enzymes.com
Tel: 1-631-562-8517 1-631-448-7888
Fax: 1-631-938-8127
Distributors To view the contact information for a specific location, select the desired country: