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Glucokinase from Human, recombinant
Cat No.
NATE-1696
Description
Glucokinase (GK; EC 2.7.1.2) is an enzyme that facilitates phosphorylation of glucose to glucose-6-phosphate. Glucokinase occurs in cells in the liver, pancreas, gut, and brain of humans and most other vertebrates. In each of these organs it plays an important role in the regulation of carbohydrate metabolism by acting as a glucose sensor, triggering shifts in metabolism or cell function in response to rising or falling levels of glucose, such as occur after a meal or when fasting. Mutations of the gene for this enzyme can cause unusual forms of diabetes or hypoglycemia.
Abbr
GCK, Recombinant (Human)
Source
E. coli
Species
Human liver
Form
Liquid
Enzyme Commission Number
EC 2.7.1.2
Activity
662 pmol/min/ug
CAS No.
9001-36-9
Molecular Weight
53.2 kDa
Purity
77% (densitometry)
Concentration
1.0mg/mL
Unit Definition
One unit is defined as the amount of enzyme that will convert 1 nmol of NADP to NADPH at 30°C.
Assay conditions: 25 mM HEPES, pH 7.5, 2 nM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2 mM ATP, 25 mM glucose, 100 ug/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human liver glucokinase at 30°C for 30 min.
Assay conditions: 25 mM HEPES, pH 7.5, 2 nM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2 mM ATP, 25 mM glucose, 100 ug/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human liver glucokinase at 30°C for 30 min.
Storage
at -80 °C
Buffer
50 mM potassium phosphate pH-7.4, 50 mM sodium chloride, 0.5 mM ethylenediaminetetraaceticacid, and 2.5% glycerol.
Synonyms
EC 2.7.1.2; glucokinase; glucokinase (phosphorylating); 9001-36-9; GCK; FGQTL3; GK; GLK; HHF3; HK4; HKIV; HXKP; LGLK; MODY2
Download
Download Datasheet| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-1686 | Glucokinase from Human, recombinant | EC 2.7.1.2 | 9001-36-9 | E. coli | Inquiry |
| NATE-1687 | Glucokinase from Human, recombinant | EC 2.7.1.2 | 9001-36-9 | E. coli | Inquiry |
| NATE-1697 | Glucokinase from Human, recombinant | EC 2.7.1.2 | 9001-36-9 | E. coli | Inquiry |
| NATE-1903 | Native Zymomonas mobilis Glucokinase | EC 2.7.1.2 | 9001-36-9 | Zymomonas mobilis | Inquiry |