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Glycerol 3-phosphate oxidase from microbial source, Recombinant

Cat No.
NATE-1616
Description
In enzymology, a glycerol-3-phosphate oxidase (EC 1.1.3.21) is an enzyme that catalyzes the chemical reaction:sn-glycerol 3-phosphate + O2↔ glycerone phosphate + H2O2. Thus, the two substrates of this enzyme are sn-glycerol 3-phosphate and O2, whereas its two products are glycerone phosphate and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. This enzyme participates in glycerophospholipid metabolism. It employs one cofactor, FAD.
Abbr
GPO, Recombinant (microbial source)
Source
Microbial source
Form
Lyophilised powder
Enzyme Commission Number
EC 1.1.3.21
Activity
2.5 U/mg protein at pH 7.0 and 37°C
CAS No.
9046-28-0
Molecular Weight
~ 68,000
Isoelectric point
~ 5.0
Unit Definition
One Unit of glycerol 3- phosphate oxidase is defined as the amount of enzyme required to oxidise one µmole of DL-α-glycerophosphate to dihydroxyacetone phosphate per minute at pH 7.0.
Stability
Stable for > 2 years at -20°C.
Storage
at -20°C
Buffer
Reconstitute the enzyme in 1 mL of distilled water.
Preparation Instructions
For assay, prepare appropriate dilutions immediately prior to use, in potassium phosphate buffer (100 mM), pH 7.0 containing BSA (1 mg/mL).
Synonyms
sn-glycerol-3-phosphate:oxygen 2-oxidoreductase
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogDIA-154
EC No.EC 1.1.3.21
CAS No.9046-28-0
SourcePediococcus sp.
CatalogDIA-199
EC No.EC 1.1.3.21
CAS No.9046-28-0
SourcePediococcus sp.
CatalogDIA-200
EC No.EC 1.1.3.21
CAS No.9046-28-0
SourceMicroorganism
CatalogDIA-286
EC No.
CAS No.9046-28-0
SourceE. coli
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Realted Protocols
GLYCEROL-3-PHOSPHATE OXIDASE -Enzymatic Assay Protocol
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