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Glycerol-3-phosphate Oxidase from E. coli, Recombinant

Cat No.
DIA-286
Description
Recombinant oxidoreductase that catalyzes the interconversion of glycerol 3-phosphate to dihydroxyacetone phosphate. Rely on the proven diagnostic quality of this product.
Abbr
GPO, Recombinant (E. coli)
Alias
GPO
Source
E. coli
Applications
Use Glycerol-3-phosphate Oxidase in diagnostic tests for the determination of triglycerides together with Glycerol Kinase and Lipoprotein Lipase.
Appearance
Greenish yellow lyophilizate
Activity
>90 U/mg lyophilizate (+37°C)
CAS No.
9046-28-0
Contaminants
Cholesterol oxidase: <0.001 Lactate oxidase: <0.002 Uricase: <0.001
Molecular Weight
75 kD (SDS-PAGE); 74 kD (gel filtration, Sephadex G 150)
Isoelectric point
~4.2
pH Stability
6.5-8.5
Michaelis Constant
K-phosphate buffer, 0.1 mol/l; pH 7.5: 1.36 x 10-2 mol/l (o-dianisidine assay) Tris buffer, 0.1 mol/l; pH 7.6: 2.90 x 10-3 mol/l (o-dianisidine assay) Tris buffer, 0.1 mol/l; pH 8.1: 1.40 x 10-3 mol/l (PAP assay)
Specificity
Glycerol phosphate oxidase reacts highly specific with L-α-glycerol phosphate.
Optimum pH
8.0-8.5
Stability
At +2 to +8°C within specification range for 12 months. Store dry.
Inhibitors
Ag, Hg-salts and SDS
Synonyms
glycerol-3-phosphate oxidase; sn-glycerol-3-phosphate: oxygen 2-oxidoreductase; glycerol phosphate oxidase; glycerol-1-phosphate oxidase; glycerol phosphate oxidase; L-alpha-glycerophosphate oxidase; alpha-glycerophosphate oxidase; L-alpha-glycerol-3-phosphate oxidase; GPO
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