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Granzyme B from Mouse, Recombinant


Cat No.
NATE-0333
Description
Granzyme B, a 247 amino acid polypeptide, contains a leader sequence, which is cleaved by a signal peptidase and a two amino acid prodomain, which is cleaved by the lysosomal cysteine protease DPPI. The recombinant granzyme B is expressed in Pichia pastoris as the mature form and appears on SDS-PAGE as a triplet (~34, 32, and 30 kDa) due to three different glycosylations. Granzyme B, a serine protease, is the most prominent granzyme in a family of 11 found in the cytotoxic granules. The granzymes enter the target cell with the assistance of perforin, a critical molecule of the cytotoxic granules. In the target cell, the granzymes act on specific substrates involved with the cell death via apoptosis.
Abbr
GzmB, Recombinant (Mouse)
Alias
GzmB; CTLA1; CCPII
Source
Pichia pastoris
Species
Mouse
Product Overview
> 90% (SDS-PAGE), recombinant, expressed in Pichia pastoris, buffered aqueous solution. Pichia expressed recombinant Granzyme B has the advantage over native Granzyme B as the latter can be purified only in limited amounts, and over E. coli and vaccinia virus expressed enzymes that failed to generate soluble active enzyme.
Form
buffered aqueous solution
Activity
> 10,000 units/mg protein
CAS No.
143180-74-9
Molecular Weight
28.9 kDa
Specificity
> 90% (SDS-PAGE)
Unit Definition
One unit will hydrolyze 1.0 nmole of Boc-Ala-Ala-Asp-sBzl per min at 30°C.
Storage
−70°C
Pathway
Activation, myristolyation of BID and translocation to mitochondria, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Apoptosis, organism-specific biosystem; Apoptosis, organism-specific biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem
Function
catalytic activity; hydrolase activity; peptidase activity; protein binding; serine-type endopeptidase activity; serine-type peptidase activity
Synonyms
granzyme B; CTLA1; CCPII; cytotoxic cell proteinase-1; granzyme G; granzyme H; CCP1 proteinase; GzmB
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