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HIV-1 Protease, Recombinant

Cat No.
NATE-1662
Description
HIV-1 protease is a retroviral aspartyl protease (retropepsin) that is essential for the life-cycle of HIV. HIV protease cleaves newly synthesized viral polyproteins at the appropriate places into functional protein products as mature protein components of an infectious HIV virion. The mutation of HIV protease's active site or inhibition of its activity disrupts HIV’s ability to replicate and infect additional cells.
Abbr
HIV-1 Protease, Recombinant
Source
E. coli
Form
Liquid
Enzyme Commission Number
EC 3.4.23.-
Activity
> 1mU/mg
Molecular Weight
38.4 kDa (1-99 aa + N-terminal GST and C-terminal Poly-his tags). It runs at ~31 kDa during SEC and SDS-PAGE analyses.
Purity
> 90%
Stability
Stable for at least 6 months as supplied. It can be further diluted to 0.5-1 mg/ml with 50 mM Sodium acetate, 100 mM NaCl, 5 mM DTT, 5 mM EDTA, pH 5.0 containing 10% glycerol, and stored at –80°C in aliquots. Do not keep the enzyme at 4°C or -20°C for extended time. Avoid repeated freezing and thawing cycles.
Storage
Store at -80°C
Synonyms
HIV-1 retropepsin; HIV-1 Protease (PR); cd05482
Catalog Product Name EC No. CAS No. Source Price
NATE-1661 HIV-2 Protease, Recombinant EC 3.4.23.- E. coli Inquiry
NATE-0344 HIV Protease Mutant, Recombinant EC 3.4.23.16 144114-21-6 E. coli Inquiry
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