Description
Heparin-degrading lyase that recognizes heparin sulfate proteoglycan as its primary substrate. Heparinase I and III plays vital role in various biological processes: modulate cell-growth factor interactions, cell-lipoprotein interactions, neovascularization. It cleaves highly sulphated polysaccharide chains in presence of 2-O-sulfated α-L-idopyranosyluronic acid and β-D-glucopyranosyluronic acid residues of polysaccharides.
Species
Flavobacterium heparinum
Activity
> 15 IU/mg (heparin as substrate), > 18 IU/mg (heparan sulfate as substrate)
Specificity
Heparin; heparan sulfate.
Unit Definition
One international unit (IU) of recombinant heparinase II is defined as the amount of enzyme that will liberate 1.0 μmole unsaturated oligosaccharides from porcine mucosal heparin per minute at 35 °C and pH 7.0.
Stability
Expiration of heparinase II is 12 months from manufacturing date, frozen at -96 to -20 °C in PBS.
Synonyms
Heparinase; Heparin lyase II; Heparinase II; 149371-12-0
