Hexosaminidase 3A from Thermotoga neapolitana, Recombinant

Cat No.

NATE-1453

Description

Hexosaminidase, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates. The activity of β-N-actylglucosaminidase may be determined with the chromogenic substrate p-nitrophenyl-N-acetyl-β-D-glucosaminide. β-N-actylglucosaminidase hydrolyzes the terminal nonreducing N-acetyl-D-hexosamine residues. This enzyme contains two predominant isozymes, Hex A, a heterodimer, and Hex B, a homodimer. N-acetylglucosamine, acetamide, N-2-acetamido-2-deoyglucosylamine, N-acetylnojirimycin, and N-acetyldeoxynojirmycin are known inhibitors.

Abbr

Hexosaminidase 3A, Recombinant (Thermotoga neapolitana)

Source

E. coli

Species

Thermotoga neapolitana

Form

35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol

Enzyme Commission Number

EC 3.2.1.52

CAS No.

9012-33-3

Molecular Weight

40.6 kDa

Purity

>90% by SDS-PAGE

Concentration

0.25 mg/mL

Specificity

Chitobiose and chitooligosaccharides

Optimum pH

7.0-8.0

Optimum temperature

65-75 °C

Storage

This enzyme is shipped at room temperature but should be stored at -20 °C.

Synonyms

EC 3.2.1.52; 9012-33-3; hexosaminidase; β-acetylaminodeoxyhexosidase; N-acetyl-β-D-hexosaminidase; N-acetyl-beta-hexosaminidase; β-hexosaminidase; β-acetylhexosaminidinase; β-D-N-acetylhexosaminidase; β-N-acetyl-D-hexosaminidase; β-N-acetylglucosaminidase; hexosaminidase A; N-acetylhexosaminidase; β-D-hexosaminidase

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