Description
Hyaluronate lyase degrades hyaluronate using an elimination reaction to break glycosidic linkages yielding unsaturated disaccharide products.
Abbr
Hyaluronate Lyase, Recombinant (Streptococcus equi)
Source
Streptococcus equi 4047
Form
Supplied in 3.2 M ammonium sulphate
Enzyme Commission Number
EC 4.2.2.1
Molecular Weight
38447.1 Da
Purity
> 95 % as judged by SDS-PAGE
Unit Definition
One unit is defined as the amount of enzyme required to release 1μmol of 4,5-unsaturated product per minute from 1 mg/mL hyaluronic acid in 50 mM sodium acetate buffer, pH 6.0, at 37°C, as measured at 232 nm.
Storage
Store at 4°C (shipped at room temperature)
Preparation Instructions
Agitate vial sufficiently to fully homogenise enzyme precipitate before use.
Synonyms
hyaluronidase; glucuronoglycosaminoglycan lyase; spreading factor; mucinase; 37259-53-3; EC 4.2.2.1; hyaluronate lyase
