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Hyaluronate lyase (novel specificity) from Soil prokaryote, Recombinant

Cat No.
NATE-1098
Description
Hyaluronate lyase degrades hyaluronate using an elimination reaction to break glycosidic linkages yielding unsaturated disaccharide products.
Abbr
Hyaluronate Lyase, Recombinant (Soil prokaryote)
Source
Soil prokaryote
Applications
High purity recombinant Hyaluronate lyase (novel specificity) (soil prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 4.2.2.1
Activity
83 U/mg protein (on Hyaluronic acid) at pH 4.2 and 25°C.
CAS No.
37259-53-3
Molecular Weight
~ 82,914
Isoelectric point
~ 6.5
Unit Definition
One Unit of hyaluronate lyase activity is defined as the amount of enzyme required to release one μmole of 4,5-unsaturated product per minute from hyaluronic acid (0.58 mM) in the presence of calcium chloride (0.1mM) in sodium succinate buffer (20 mM) pH 4.2 and 25°C, monitored at 232 nm.
Optimum pH
4.2
Thermal stability
up to 25°C
Storage
4°C
Preparation Instructions
For assay, this enzyme should be diluted in sodium succinate buffer (20 mM) pH 4.2 containing 0.5 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Synonyms
hyaluronidase; glucuronoglycosaminoglycan lyase; spreading factor; mucinase; 37259-53-3; EC 4.2.2.1; hyaluronate lyase
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