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Isopullulanase from Aspergillus niger, Recombinant

Cat No.
NATE-0850
Description
Isopullulanase (EC 3.2.1.57) is an enzyme with system name pullulan 4-glucanohydrolase (isopanose-forming). This enzyme catalyses the following chemical reaction: Hydrolysis of pullulan to isopanose (6-alpha-maltosylglucose). The enzyme has no activity on starch.
Abbr
Isopullulanase, Recombinant (Aspergillus niger)
Source
Aspergillus niger
Applications
High purity recombinant Isopullulanase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
In 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.2.1.57
Activity
42 U/mg protein (on pullulan) at pH 3.5 and 40°C.
CAS No.
37288-43-0
Molecular Weight
~ 60.5 kDa
Isoelectric point
~ 5.2
pH Stability
3.0 - 9.0 (> 75% control activity after 24 hours at 4°C)
Specificity
Hydrolysis of pullulan to isopanose (6-α-maltosylglucose).
Unit Definition
One Unit of isopullulanase activity is defined as the amount of enzyme required to release one µmole of glucose reducing sugar equivalents per minute from pullulan (10 mg/mL) in formic acid buffer (100 mM), pH 3.5 at 40°C.
Optimum pH
3.0 - 3.5
Optimum temperature
40°C
Stability
> 2 years at 4°C.
Storage
Store at 4°C.
Buffer
0.02% (w/v) sodium azide
Preparation Instructions
For assay, this enzyme should be diluted in formic acid buffer (100 mM), pH 3.5 containing 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Synonyms
Isopullulanase; EC 3.2.1.57; pullulan 4-glucanohydrolase (isopanose-forming)
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EC No.EC 3.2.1.57
CAS No.37288-43-0
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